2

Statistics for ChIP-chip and DNase hypersensitivity experiments on NimbleGen arrays

Data obtained from high-density oligonucleotide tiling arrays present new computational challenges for users. This chapter presents ACME (Algorithm for Capturing Microarray Enrichment), a computer program developed for the analysis of data obtained …

Gene expression profiling of human sarcomas: insights into sarcoma biology

Sarcomas are a biologically complex group of tumors of mesenchymal origin. By using gene expression microarray analysis, we aimed to find clues into the cellular differentiation and oncogenic pathways active in these tumors as well as potential …

BioMart and Bioconductor: a powerful link between biological databases and microarray data analysis

biomaRt is a new Bioconductor package that integrates BioMart data resources with data analysis software in Bioconductor. It can annotate a wide range of gene or gene product identifiers (e.g. Entrez-Gene and Affymetrix probe identifiers) with …

Database of mRNA gene expression profiles of multiple human organs

Genome-wide expression profiling of normal tissue may facilitate our understanding of the etiology of diseased organs and augment the development of new targeted therapeutics. Here, we have developed a high-density gene expression database of 18,927 …

Impact of overlapping recruitment on linkage analysis of complex disorders: simulation studies

Evidence for significant linkage in complexly inherited disorders usually necessitates independent, replicative studies. This study investigates the implications of including in the replicative studies families already used to suggest linkage in …

Linkage and association between inflammatory bowel disease and a locus on chromosome 12

Genetic epidemiological studies have shown that genetic factors are important in the pathogenesis of the idiopathic inflammatory bowel diseases (IBD), Crohn disease (CD), and ulcerative colitis (UC). A genome screen in the United Kingdom found …

Comparison of nonparametric statistics for detection of linkage in nuclear families: single-marker evaluation

We have evaluated 23 different statistics, from a total of 10 popular software packages for model-free linkage analysis of nuclear-family data, by applying them to single-marker data simulated under several two-locus disease models. The statistics …

Genetic susceptibility for human familial essential hypertension in a region of homology with blood pressure linkage on rat chromosome 10

Hypertension is a significant risk factor for heart attack and stroke and represents a major public health burden because of its high prevalence (e.g. 15-20% of the European and American populations). Although blood pressure is known to have a strong …

Analysis of a complex oligogenic disease

Our analysis of GAW10 problem 2 data set consisted of linear regression analysis followed by linkage analysis. The linear regression analysis allowed some exploration of the relationships between the quantitative variables. Furthermore, it isolated …

Analysis of bipolar disorder using affected relatives

We have analyzed the GAW10 data from several studies of bipolar affective disorder (BPAD) using the software packages SimIBD and SIMWALK2. SimIBD implements a simulation-based affected-pedigree-member (APM) statistic, called SimAPM, as well as an …